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This study integrated thermally induced super‐resolution into near‐field photolithography and conducted simulation and analysis on line segment fabrication. This technique involves passing a laser beam through an aluminum‐plated optical fiber probe onto a thin film of indium (approximately 10 nm thick). The indium film opens a melted aperture narrower than the width of the laser beam, creating a melted...
Generally, in scanning electron microscopy (SEM) imaging, it is desirable that a high‐resolution image be composed mainly of those secondary electrons (SEs) generated by the primary electron beam, denoted SEI. However, in conventional SEM imaging, other, often unwanted, signal components consisting of backscattered electrons (BSEs), and their associated SEs, denoted SEII, are present; these signal...
The presence of residual endodontic sealer in the pulp chamber may cause discoloration of the dental crown and interfere with the adhesion of restorative materials. The aim of this study was to compare the efficacy of different solvents in removing residues of an epoxy resin‐based sealer (AH Plus) from the dentin walls of the pulp chamber, by scanning electron microscopy (SEM). Forty‐four bovine incisor...
Papulonodular mucinosis (PM) is a cutaneous clue to the presence and activity of silent lupus erythematosus (LE), but the exact pathogenesis is still under secret. Moreover, the currently available treatments for PM are not satisfactory. To demonstrate the possibility of multiphoton microscopy (MPM) to trace the pathological state of PM and evaluate the treatment efficacy, epidermal and dermal alteration...
Atomic force microscopy (AFM) provides a new technology to visualize the cellular topography and quantify the molecular interactions at nanometer spatial resolution. In this work, AFM was used to image the cellular topography and measure the molecular force of pathological cells from B‐cell lymphoma patients. After the fluorescence staining, cancer cells were recognized by their special morphological...
Antitumor immunotherapies, as a prospective approach for local cancer treatment, are attracting increasing interests. To detect the reacting course of immune and tumor cells, we have observed the process of K562 cells (a human erythroleukemic cell line) coculturing with peripheral lymphocytes, and the morphological and ultrastructural alterations of K562 cells and lymphocytes were investigated as...
Although previous use‐wear studies involving quartz and quartzite have been undertaken by archaeologists, these are comparatively few in number. Moreover, there has been relatively little effort to quantify use‐wear on stone tools made from quartzite. The purpose of this article is to determine the effectiveness of a measurement system, laser scanning confocal microscopy (LSCM), to document the surface...
Cantilever‐based optical interfacial force microscopy (COIFM) was applied to the investigation of the mechanical properties of soft materials to avoid the double‐spring effect and snap‐to‐contact problem associated with atomic force microscopy (AFM). When a force was measured as a function of distance between an oxidized silicon probe and the surface of a soft hydrocarbon film, it increases nonlinearly...
Second harmonic microscopic imaging and spectroscopy technology has become a powerful tool for biomedical studies, especially in fibrosis‐related diseases research. And type I collagen is the major risk factors for fibrotic diseases. In this study, model for three‐dimensional (3‐D) uniform arrangement type I collagen is set up for researching the second harmonic generation (SHG) on nonlinear optics...
Pyramidal nanoindentation loading curves were linearly plotted, normal force versus (penetration depth)3/2. The slope is penetration resistance k, its inverse penetrability. Linear correlations verify. All contributions to the indentation are included in the penetrability. Dependencies and uses of the extrapolation tools are exemplified, identified, and discussed. In the case of phase transition including...
The integrity and adhesion properties of endothelium play vital roles during atherosclerosis. It is well known that oxidized low‐density lipoprotein (Ox‐LDL) influences many physiological activities or mechanical properties of endothelial cells. However, the effects of Ox‐LDL on the integrity and nonspecific adhesion properties of endothelial cells are still unclear. In this study, using the topographical...
The single‐file root canal instrumentation technique using reciprocating motion has been gaining concern. Therefore, the purpose of this study was to compare the shaping ability of single ProTaper F2 file and WaveOne Primary file when they were used in the curved root canal with reciprocation motion and to investigate the durability of the file after use with a scanning electron microscopy (SEM)....
In semiconductor industry, strict critical dimension control by using a critical dimension scanning electron microscope (CD‐SEM) is an extremely urgent task in near‐term years. A Monte Carlo simulation model for study of CD‐SEM image has been established, which is based on using Mott's cross section for electron elastic scattering and the full Penn dielectric function formalism for electron inelastic...
Detection of cracks from stainless steel pipe images is done using contrast stretching technique. The technique is based on an image filter technique through mathematical morphology that can expose the cracks. The cracks are highlighted and noise removal is done efficiently while still retaining the edges. An automated crack detection system with a camera platform has been successfully implemented...
An electron beam inducing method for sprouting large quantities of silver nanoparticles on the surface of silver chloride particles is reported. The electron beam driven process was characterized by time‐dependent scanning electron microscope (SEM) and energy dispersive spectrum (EDS), allowing for observing several key intermediates in and characteristics of the growth process. Theoretical calculation...
Although bone minerals have been widely studied by various techniques in previous studies, crystal structures, morphology of bone minerals and its building pathway remained still controversy. In this work, the ultrastructure of the mineralization front of rabbit femur has been studied by conventional and high‐resolution (HR) transmission electron microscopy (TEM). In order to induce a healing and...
Scanning electron microscopy/energy dispersive X‐ray spectrometry (SEM/EDS) is a widely applied elemental microanalysis method capable of identifying and quantifying all elements in the periodic table except H, He, and Li. By following the “k‐ratio” (unknown/standard) measurement protocol development for electron‐excited wavelength dispersive spectrometry (WDS), SEM/EDS can achieve accuracy and precision...
Focused ion beam nanotomography‐scanning electron microscopy tomography yields high‐quality three‐dimensional images of materials microstructures at the nanometer scale combining serial sectioning using a focused ion beam with SEM. However, FIB‐SEM tomography of highly porous media leads to shine‐through artifacts preventing automatic segmentation of the solid component. We simulate the SEM process...
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